GAGA Assembly Progress Report 2021
1 Overview of GAGA assemblies
1.1 Taxon sampling
We so far have genomic data for 163 species distributed across the 347 genera of ants (Fig 1.1), covering 27 tribes in 12 subfamilies. Myrmicinae are represented by 77 genomes, followed by Formicinae and Ponerinae with 39 and 21 genomes sequenced, respectively. With regard to the tribe, Crematogastrini make up the largest group with 35 genomes, followed by Attini and Ponerini with 25 and 20 genomes sequenced, respectively.
Figure 1.1: Ant genera targeted so far by GAGA. The Figure shows all extant genera of the Formicidae with the corresponding species sequenced by GAGA for the different genera.
1.2 Sequencing techniques
Most (n= 125) of the species have been sequenced with PacBio, while some rare species (e.g. Leptanilla) could only be sequenced with stLFR (n= 20), commonly due to lack of sufficient biomass (Fig. 1.2). HiC sequencing was so far done for 15 species, allowing us to assemble chromosome-scale genomes for 18 species. Species highlighted in grey in the phylogeny were not assembled by GAGA but included in our comparative analyses as they met our quality criteria.
Figure 1.2: Sequencing techniques for different species. The left panel shows the techniques combined for genome sequencing of the different species. The right panel shows the coverage of RNAseq data, divided by caste, sex, and developmental stage.
1.3 Genome completeness
Most genomes reach high BUSCO scores (Fig 1.3), both against Hymenoptera (median 97 %) and Eukaryota (median 98 %). A few stLFR-only genomes however are incomplete, with BUSCO scores as low as ~70 % (e.g. Gigantiops destructor with 68.3 % complete Hymenoptera BUSCOs).
We have now also finalized the gene annotations for all species. On average, we annotated 13182 genes (range 10973 to 16903). BUSCO scores for the protein annotations were highly correlated with scores for the unannotated genome, indicating that our gene annotation approach is working well.
Figure 1.3: BUSCO scores for all target species. The first column shows completeness scores for genome assemblies against Hymenoptera BUSCOs, the second column shows completeness scores for genome assemblies against Eukaryota BUSCOs, the third column shows completeness scores for protein coding gene annotations against Hymenoptera BUSCOs. The top inset plot shows the correlation of BUSCO scores for ‘genome’ and ‘protein’ mode. The bottom inset shows the annotation completeness by sequencing technique. P=PacBio, H=HiC, s=short-read (stLFR or wgs).
1.4 Genome sizes of all so far sequenced species
The assembled genomes vary considerably in size, ranging from 188.61 MB (Cardiocondyla obscurior) to 594.51 MB (Odontomachus cf. monticola). Overall, genome size tends to vary most in the Ponerinae and least in the Formicinae. Figure 1.4 gives a complete overview of all so far sequenced species and the respective genome assembly size in Mb.
Figure 1.4: Genome sizes of GAGA genome assemblies
1.5 Preliminary quality of the assembled genomes
Our sequencing strategy has evolved further, now combining long-read PacBio data, linked-short-read stLFR data and if possible HiC data. We expect that together, these data will yield highly contiguous high quality genome assemblies that should be close to chromosome-resolution for most of the species.
Figure 1.5 gives a rough overview of the quality of the genome assemblies we have so far.
On average, scaffold N50 is 4.55 mega bases (Mb) Currently, the species with the highest scaffold N50 is Aphaenogaster subterranea with 35.8 MB.
Figure 1.5: Genome assembly quality for GAGA species.
Figure 1.6: Genome assembly quality overview for GAGA species.
1.6 Overview Table
Table 1.1 gives an overview of all so far sequenced species.
| GAGA-id | Taxon | Tribe | Subfamily | Sequencing Technique | Genome Size | %BUSCO (Hym) | %BUSCO (Euk) | N50scf | Assembly Status |
|---|---|---|---|---|---|---|---|---|---|
| GAGA-0392 | Leptanilla sp. | Leptanillini | Leptanillinae | stLFR | 224.4382 | 93.3 | 98.0 | 0.13 | Final |
| GAGA-0534 | Dorylus orientalis | tr. Dorylinae | Dorylinae | P/s | 190.0425 | 96.3 | 98.4 | 14.28 | Final |
| GAGA-0577 | Parasyscia sp. | tr. Dorylinae | Dorylinae | stLFR | 241.0862 | 78.6 | 87.5 | 0.02 | Final |
| NCBI-0001 | Ooceraea biroi | tr. Dorylinae | Dorylinae | non-GAGA | 223.8342 | 97.4 | 97.7 | 16.89 | NCBI assembly |
| GAGA-0379 | Cerapachys sulcinodis | tr. Dorylinae | Dorylinae | P/s | 314.9558 | 96.8 | 97.7 | 2.37 | Final |
| GAGA-0365 | Tetraponera rufonigra | tr. Pseudomyrmecinae | Pseudomyrmecinae | P/s | 250.8015 | 95.3 | 98.1 | 9.03 | Final |
| GAGA-0343 | Pseudomyrmex spinicola | tr. Pseudomyrmecinae | Pseudomyrmecinae | P/s | 263.0094 | 96.9 | 98.1 | 4.52 | Final |
| GAGA-0517 | Myrmecia pilosula | Myrmeciini | Myrmeciinae | P/s | 219.4548 | 98.1 | 98.1 | 11.50 | Final |
| GAGA-0521 | Myrmecia croslandi 2n=2 | Myrmeciini | Myrmeciinae | P/s | 189.4173 | 96.6 | 96.1 | 26.09 | Final |
| GAGA-0522 | Myrmecia croslandi 2n=4 | Myrmeciini | Myrmeciinae | P/s | 195.9851 | 98.1 | 98.8 | 21.92 | Final |
| GAGA-0528 | Liometopum microcephalum | Tapinomini | Dolichoderinae | P/s | 230.3274 | 96.3 | 96.4 | 1.22 | Final |
| GAGA-0340 | Tapinoma cf. melanocephalum | Tapinomini | Dolichoderinae | P/s | 261.1961 | 94.6 | 96.1 | 9.68 | Final |
| GAGA-0337 | Tapinoma melanocephalum | Tapinomini | Dolichoderinae | P/s | 254.7987 | 95.4 | 97.6 | 6.18 | Final |
| NCBI-0010 | Linepithema humile | Leptomyrmecini | Dolichoderinae | non-GAGA | 219.4721 | 97.2 | 98.9 | 1.40 | NCBI assembly |
| GAGA-0363 | Iridomyrmex anceps | Leptomyrmecini | Dolichoderinae | P/s | 223.6939 | 95.7 | 96.1 | 9.59 | Final |
| GAGA-0302 | Ochetellus glaber | Leptomyrmecini | Dolichoderinae | P/s | 300.0546 | 96.2 | 97.3 | 3.64 | Final |
| GAGA-0026 | Myrmecocystus cf. mendax | Lasiini | Formicinae | P/H | 220.3007 | 98.1 | 99.2 | 14.29 | Final |
| GAGA-0366 | Lasius neglectus | Lasiini | Formicinae | stLFR | 254.7609 | 92.7 | 97.3 | 0.03 | Final |
| GAGA-0177 | Lasius niger | Lasiini | Formicinae | P/H/s | 287.3905 | 96.9 | 96.5 | 19.67 | Final |
| GAGA-0532 | Lasius alienus | Lasiini | Formicinae | P/s | 287.4464 | 97.8 | 98.8 | 3.29 | Final |
| GAGA-0491 | Lasius fuliginosus | Lasiini | Formicinae | P/s | 239.9295 | 97.7 | 99.2 | 2.37 | Final |
| GAGA-0524 | Lasius fuliginosus | Lasiini | Formicinae | P/s | 238.8692 | 97.9 | 99.2 | 1.65 | Final |
| GAGA-0364 | Lasius sp1 | Lasiini | Formicinae | P/s | 250.7462 | 98.0 | 99.6 | 3.58 | Final |
| GAGA-0024 | Lasius flavus | Lasiini | Formicinae | P/H/s | 300.4456 | 97.6 | 98.8 | 18.92 | Final |
| GAGA-0020 | Lasius flavus sp.2 | Lasiini | Formicinae | P/H | 320.2268 | 95.2 | 95.7 | 5.72 | Final |
| GAGA-0341 | Nylanderia fulva | Lasiini | Formicinae | PacBio | 336.7758 | 97.1 | 98.8 | 2.58 | Final |
| GAGA-0332 | Pseudolasius sp. | Lasiini | Formicinae | P/s | 265.1858 | 95.8 | 96.9 | 4.62 | Final |
| GAGA-0198 | Paratrechina longicornis | Lasiini | Formicinae | P/s | 247.5501 | 97.2 | 98.5 | 11.98 | Final |
| GAGA-0275 | Euprenolepis cf wittei | Lasiini | Formicinae | P/s | 265.7238 | 97.7 | 99.2 | 2.38 | Final |
| GAGA-0055 | Gigantiops destructor | Gigantiopini | Formicinae | stLFR | 241.2114 | 68.3 | 82.8 | 0.01 | Final |
| GAGA-0406 | Myrmoteras binghamii | Myrmoteratini | Formicinae | stLFR | 282.7553 | 73.0 | 88.3 | 0.01 | Final |
| GAGA-0304 | Proformica cf. mongolica | Formicini | Formicinae | P/s | 256.7547 | 95.4 | 94.9 | 4.70 | Final |
| GAGA-0354 | Cataglyphis aenescens | Formicini | Formicinae | P/s | 278.8879 | 97.7 | 98.4 | 5.34 | Final |
| GAGA-0494 | Rossomyrmex minuchae | Formicini | Formicinae | P/s | 253.1488 | 97.0 | 97.7 | 5.31 | Final |
| GAGA-0334 | Rossomyrmex quandratinodum | Formicini | Formicinae | P/s | 250.9996 | 97.5 | 98.8 | 5.05 | Final |
| GAGA-0502 | Iberoformica subrufa | Formicini | Formicinae | P/s | 289.9265 | 97.9 | 97.7 | 4.81 | Final |
| GAGA-0485 | Formica sanguinea | Formicini | Formicinae | P/s | 348.2435 | 97.6 | 99.6 | 3.98 | Final |
| NCBI-0008 | Formica exsecta | Formicini | Formicinae | non-GAGA | 274.6670 | 96.2 | 98.4 | 1.01 | NCBI assembly |
| OUT-0001 | Formica selysi | Formicini | Formicinae | non-GAGA | 290.1511 | 96.9 | 98.0 | 7.92 | NCBI assembly |
| OUT-0002 | Formica cinerea | Formicini | Formicinae | PacBio | 324.0927 | 98.2 | 98.9 | 5.06 | Final |
| GAGA-0495 | Formica fusca | Formicini | Formicinae | stLFR | 245.6066 | 94.4 | 97.7 | 0.13 | Final |
| GAGA-0350 | Formica fusca | Formicini | Formicinae | P/s | 333.3169 | 95.2 | 94.5 | 2.18 | Final |
| GAGA-0359 | Formica cf. japonica | Formicini | Formicinae | P/s | 388.7580 | 96.6 | 97.6 | 1.78 | Final |
| GAGA-0336 | Oecophylla smaragdina | Oecophyllini | Formicinae | P/s | 218.7865 | 97.5 | 98.8 | 7.71 | Final |
| GAGA-0199 | Anoplolepis gracilipes | Plagiolepidini | Formicinae | P/s | 253.3809 | 98.1 | 99.2 | 9.15 | Final |
| GAGA-0187 | Plagiolepis pygmaea | Plagiolepidini | Formicinae | P/H/s | 334.6678 | 96.6 | 97.7 | 32.91 | Final |
| GAGA-0338 | Lepisiota rothneyi | Plagiolepidini | Formicinae | P/s | 351.2237 | 96.7 | 97.7 | 2.55 | Final |
| GAGA-0360 | Colobopsis sp. | Camponotini | Formicinae | P/s | 262.4213 | 98.3 | 99.2 | 9.35 | Final |
| GAGA-0374 | Polyrhachis illaudata | Camponotini | Formicinae | P/s | 274.1765 | 97.8 | 96.9 | 2.41 | Final |
| GAGA-0200 | Camponotus japonicus | Camponotini | Formicinae | P/s | 305.8157 | 98.0 | 97.7 | 1.49 | Final |
| GAGA-0396 | Camponotus singularis | Camponotini | Formicinae | P/s | 264.1384 | 98.0 | 98.4 | 3.46 | Final |
| NCBI-0005 | Camponotus floridanus | Camponotini | Formicinae | non-GAGA | 284.0092 | 98.5 | 98.4 | 1.59 | NCBI assembly |
| GAGA-0362 | Camponotus sp. | Camponotini | Formicinae | P/s | 283.8616 | 97.8 | 99.2 | 8.31 | Final |
| GAGA-0361 | Camponotus cf. fedtschenkoi | Camponotini | Formicinae | P/s | 252.3328 | 98.0 | 98.0 | 9.40 | Final |
| GAGA-0221 | Camponotus fellah | Camponotini | Formicinae | P/H/s | 278.1866 | 98.0 | 98.4 | 12.30 | Final |
| GAGA-0306 | Gnamptogenys bicolor | Ectatommini | Ectatomminae | P/s | 320.0701 | 96.1 | 97.3 | 5.06 | Final |
| GAGA-0234 | Typhlomyrmex rogenhoferi | Typhlomyrmecini | Ectatomminae | stLFR | 376.5309 | 91.3 | 94.5 | 0.08 | Final |
| NCBI-0012 | Pogonomyrmex barbatus | Pogonomyrmecini | Myrmicinae | non-GAGA | 235.6213 | 93.4 | 95.7 | 0.82 | NCBI assembly |
| GAGA-0114 | Manica rubida | Myrmicini | Myrmicinae | P/H/s | 331.0788 | 95.5 | 98.9 | 11.52 | Final |
| GAGA-0401 | Myrmica sp. | Myrmicini | Myrmicinae | P/s | 474.2606 | 96.5 | 97.7 | 0.71 | Final |
| GAGA-0028 | Myrmica rubra | Myrmicini | Myrmicinae | P/H/s | 477.0295 | 95.2 | 97.3 | 1.45 | Final |
| GAGA-0087 | Myrmica scabrinodis | Myrmicini | Myrmicinae | P/s | 477.9174 | 97.0 | 98.9 | 1.66 | Final |
| GAGA-0109 | Stenamma debile | Stenammini | Myrmicinae | P/H/s | 380.3484 | 96.7 | 96.5 | 9.27 | Final |
| GAGA-0505 | Goniomma hispanicum | Stenammini | Myrmicinae | P/s | 290.1660 | 98.2 | 98.5 | 13.80 | Final |
| GAGA-0503 | Oxyopomyrmex saulcyi | Stenammini | Myrmicinae | P/s | 274.4092 | 98.3 | 98.8 | 10.82 | Final |
| GAGA-0413 | Messor capitatus | Stenammini | Myrmicinae | P/s | 304.9323 | 98.3 | 98.4 | 11.42 | Final |
| GAGA-0004 | Messor barbarus | Stenammini | Myrmicinae | P/H/s | 312.7939 | 97.3 | 98.1 | 13.01 | Final |
| GAGA-0084 | Aphaenogaster subterranea | Stenammini | Myrmicinae | P/s | 415.4753 | 97.7 | 98.5 | 35.80 | Final |
| GAGA-0356 | Aphaenogaster exasperata | Stenammini | Myrmicinae | P/s | 398.4363 | 98.0 | 98.4 | 4.32 | Final |
| GAGA-0531 | Aphaenogaster famelica | Stenammini | Myrmicinae | P/s | 368.5086 | 96.8 | 96.1 | 1.63 | Final |
| NCBI-0013 | Vollenhovia emeryi | Crematogastrini | Myrmicinae | non-GAGA | 286.6831 | 97.5 | 98.5 | 1.35 | NCBI assembly |
| GAGA-0515 | Cardiocondyla obscurior | Crematogastrini | Myrmicinae | PacBio | 188.6064 | 98.7 | 98.9 | 5.74 | Final |
| GAGA-0405 | Tetramorium sp. | Crematogastrini | Myrmicinae | P/s | 262.3968 | 97.3 | 99.2 | 5.15 | Final |
| GAGA-0553 | Tetramorium sp. | Crematogastrini | Myrmicinae | stLFR | 238.2826 | 87.1 | 95.3 | 0.03 | Final |
| GAGA-0333 | Tetramorium bicarinatum | Crematogastrini | Myrmicinae | P/s | 261.0466 | 97.6 | 98.8 | 11.33 | Final |
| GAGA-0527 | Tetramorium “Anergates” atratulus | Crematogastrini | Myrmicinae | P/s | 214.7570 | 96.9 | 98.8 | 16.30 | Final |
| GAGA-0085 | Tetramorium caespitum | Crematogastrini | Myrmicinae | P/s | 257.5558 | 96.5 | 96.9 | 8.26 | Final |
| GAGA-0520 | Melissotarsus emeryi | Crematogastrini | Myrmicinae | P/s | 386.5540 | 95.6 | 97.7 | 6.49 | Final |
| GAGA-0533 | Carebara sp. | Crematogastrini | Myrmicinae | P/s | 194.4953 | 98.5 | 99.6 | 7.60 | Final |
| GAGA-0378 | Carebara bengalensis | Crematogastrini | Myrmicinae | PacBio | 209.4950 | 95.7 | 95.7 | 6.15 | Final |
| GAGA-0578 | Carebara capreola | Crematogastrini | Myrmicinae | PacBio | 229.3550 | 96.4 | 96.5 | 5.07 | Final |
| GAGA-0331 | Carebara sp. s2 | Crematogastrini | Myrmicinae | stLFR | 214.4154 | 85.5 | 93.0 | 0.04 | Final |
| GAGA-0579 | Carebara melasolena | Crematogastrini | Myrmicinae | PacBio | 201.6361 | 97.6 | 98.9 | 5.22 | Final |
| GAGA-0328 | Carebara diversa | Crematogastrini | Myrmicinae | P/s | 199.6166 | 98.4 | 99.2 | 4.85 | Final |
| GAGA-0382 | Carebara trechideros | Crematogastrini | Myrmicinae | P/s | 200.7891 | 98.5 | 99.6 | 8.19 | Final |
| GAGA-0165 | Eutetramorium mocquerysi | Crematogastrini | Myrmicinae | P/s | 237.0488 | 98.3 | 98.8 | 9.23 | Final |
| GAGA-0407 | Kartidris sp. | Crematogastrini | Myrmicinae | P/s | 238.1303 | 96.4 | 96.9 | 6.19 | Final |
| GAGA-0335 | Meranoplus cf. bicolor | Crematogastrini | Myrmicinae | P/s | 253.9013 | 97.3 | 98.1 | 5.87 | Final |
| GAGA-0330 | Crematogaster cf. rogenhoferi | Crematogastrini | Myrmicinae | P/s | 365.4193 | 97.5 | 97.3 | 2.29 | Final |
| GAGA-0395 | Crematogaster osakensis | Crematogastrini | Myrmicinae | P/s | 265.9456 | 96.5 | 96.1 | 5.67 | Final |
| GAGA-0393 | Crematogaster cf. osakensis | Crematogastrini | Myrmicinae | P/s | 262.9190 | 96.9 | 97.3 | 4.28 | Final |
| GAGA-0103 | Myrmecina graminicola | Crematogastrini | Myrmicinae | P/s | 308.7440 | 95.8 | 96.9 | 1.46 | Final |
| GAGA-0256 | Acanthomyrmex ferox | Crematogastrini | Myrmicinae | P/s | 292.8476 | 96.9 | 96.9 | 2.52 | Final |
| GAGA-0082 | Pristomyrmex punctatus | Crematogastrini | Myrmicinae | P/s | 291.4593 | 96.2 | 98.0 | 8.36 | Final |
| GAGA-0098 | Harpagoxenus sublaevis | Crematogastrini | Myrmicinae | PacBio | 312.9646 | 95.4 | 96.1 | 2.52 | Final |
| GAGA-0099 | Leptothorax acervorum | Crematogastrini | Myrmicinae | PacBio | 341.7882 | 95.0 | 97.3 | 2.02 | Final |
| GAGA-0463 | Formicoxenus nitidulus | Crematogastrini | Myrmicinae | P/s | 307.0933 | 97.4 | 98.4 | 4.55 | Final |
| GAGA-0222 | Temnothorax rugatulus | Crematogastrini | Myrmicinae | PacBio | 322.3900 | 95.2 | 97.3 | 1.99 | Final |
| GAGA-0511 | Temnothorax ravouxi | Crematogastrini | Myrmicinae | PacBio | 371.0627 | 94.6 | 95.7 | 0.62 | Final |
| GAGA-0224 | Temnothorax americanus | Crematogastrini | Myrmicinae | PacBio | 255.5200 | 96.2 | 97.7 | 3.89 | Final |
| GAGA-0512 | Temnothorax pilagens | Crematogastrini | Myrmicinae | PacBio | 309.5717 | 96.0 | 98.1 | 1.98 | Final |
| GAGA-0513 | Temnothorax ambiguus | Crematogastrini | Myrmicinae | PacBio | 302.6095 | 96.0 | 97.7 | 2.33 | Final |
| GAGA-0223 | Temnothorax longispinosus | Crematogastrini | Myrmicinae | PacBio | 301.5131 | 95.4 | 96.5 | 1.18 | Final |
| GAGA-0288 | Temnothorax unifasciatus | Crematogastrini | Myrmicinae | PacBio | 286.9455 | 95.5 | 95.7 | 2.09 | Final |
| GAGA-0510 | Temnothorax nylanderi | Crematogastrini | Myrmicinae | PacBio | 293.4694 | 95.2 | 97.3 | 2.16 | Final |
| GAGA-0346 | Megalomyrmex milenae | Solenopsidini | Myrmicinae | P/H/s | 352.0165 | 97.3 | 98.0 | 10.85 | Final |
| GAGA-0245 | Monomorium pharaonis | Solenopsidini | Myrmicinae | P/H/s | 325.5337 | 97.8 | 99.2 | 29.66 | Final |
| NCBI-0002 | Solenopsis invicta | Solenopsidini | Myrmicinae | non-GAGA | 413.9562 | 98.0 | 98.8 | 13.11 | NCBI assembly |
| GAGA-0454 | Solenopsis fugax | Solenopsidini | Myrmicinae | P/H/s | 383.1384 | 97.3 | 98.4 | 2.83 | Final |
| GAGA-0229 | Cephalotes cf minutus | Attini | Myrmicinae | P/s | 407.5352 | 96.2 | 98.1 | 2.79 | Final |
| GAGA-0080 | Pheidole pallidula | Attini | Myrmicinae | PacBio | 302.1115 | 97.9 | 98.9 | 4.67 | Final |
| GAGA-0358 | Pheidole nodus | Attini | Myrmicinae | P/s | 346.7517 | 97.0 | 97.3 | 5.82 | Final |
| GAGA-0376 | Pheidole capellinii | Attini | Myrmicinae | P/s | 293.0382 | 95.9 | 97.3 | 5.23 | Final |
| GAGA-0530 | Pheidole yeensis | Attini | Myrmicinae | P/s | 327.1952 | 97.7 | 99.2 | 3.35 | Final |
| GAGA-0384 | Pheidole selathorax | Attini | Myrmicinae | P/s | 274.5724 | 95.1 | 94.6 | 5.30 | Final |
| GAGA-0554 | Strumigenys mutica | Attini | Myrmicinae | stLFR | 232.5091 | 92.6 | 96.5 | 0.06 | Final |
| NCBI-0014 | Wasmannia auropunctata | Attini | Myrmicinae | non-GAGA | 305.9982 | 96.6 | 98.1 | 1.42 | NCBI assembly |
| GAGA-0543 | Kalathomyrmex emeryi | Attini | Myrmicinae | P/s | 299.3814 | 97.4 | 98.0 | 6.55 | Final |
| GAGA-0541 | Mycetosoritis hartmanni | Attini | Myrmicinae | P/s | 349.2195 | 94.8 | 96.1 | 4.66 | Final |
| NCBI-0006 | Cyphomyrmex costatus | Attini | Myrmicinae | non-GAGA | 296.9971 | 97.8 | 99.6 | 1.17 | NCBI assembly |
| GAGA-0539 | Mycetophylax morschi | Attini | Myrmicinae | P/s | 308.5390 | 98.1 | 98.4 | 7.84 | Final |
| GAGA-0538 | Sericomyrmex mayri | Attini | Myrmicinae | P/s | 395.5876 | 97.9 | 98.1 | 5.32 | Final |
| GAGA-0550 | Xerolitor explicatus | Attini | Myrmicinae | P/s | 468.8864 | 94.6 | 96.5 | 0.85 | Final |
| NCBI-0015 | Trachymyrmex zeteki | Attini | Myrmicinae | non-GAGA | 267.3237 | 97.5 | 98.0 | 1.33 | NCBI assembly |
| NCBI-0017 | Trachymyrmex cornetzi | Attini | Myrmicinae | non-GAGA | 365.1377 | 97.2 | 98.4 | 0.78 | NCBI assembly |
| NCBI-0016 | Trachymyrmex septentrionalis | Attini | Myrmicinae | non-GAGA | 290.4538 | 98.0 | 98.8 | 2.52 | NCBI assembly |
| GAGA-0014 | Acromyrmex echinatior | Attini | Myrmicinae | P/H | 296.0807 | 98.2 | 98.8 | 15.11 | Final |
| GAGA-0220 | Acromyrmex octospinosus | Attini | Myrmicinae | stLFR | 314.9204 | 86.1 | 89.0 | 0.30 | Final |
| GAGA-0001 | Acromyrmex ameliae | Attini | Myrmicinae | P/s | 289.5902 | 96.4 | 98.0 | 8.21 | Final |
| GAGA-0003 | Acromyrmex lobicornis | Attini | Myrmicinae | P/H | 334.9121 | 98.4 | 98.8 | 17.01 | Final |
| GAGA-0002 | Acromyrmex subterraneus | Attini | Myrmicinae | stLFR | 318.4256 | 80.1 | 89.8 | 0.09 | Final |
| NCBI-0004 | Atta colombica | Attini | Myrmicinae | non-GAGA | 290.8997 | 98.1 | 98.8 | 2.04 | NCBI assembly |
| NCBI-0003 | Atta cephalotes | Attini | Myrmicinae | non-GAGA | 317.6720 | 93.4 | 97.3 | 5.15 | NCBI assembly |
| GAGA-0540 | Myrmicocrypta uncinata | Attini | Myrmicinae | P/s | 441.3375 | 95.8 | 95.6 | 1.90 | Final |
| GAGA-0090 | Platythyrea punctata | Platythyreini | Ponerinae | P/s | 195.8959 | 96.8 | 98.4 | 4.08 | Final |
| GAGA-0063 | Neoponera goeldii | Ponerini | Ponerinae | P/s | 554.1786 | 95.6 | 98.0 | 8.32 | Final |
| NCBI-0007 | Dinoponera quadriceps | Ponerini | Ponerinae | non-GAGA | 258.4909 | 96.3 | 97.3 | 1.36 | NCBI assembly |
| GAGA-0351 | Diacamma rugosum | Ponerini | Ponerinae | P/s | 268.9508 | 96.5 | 98.8 | 7.84 | Final |
| GAGA-0083 | Diacamma cf. indicum | Ponerini | Ponerinae | P/s | 232.5468 | 93.0 | 93.4 | 17.25 | Final |
| GAGA-0300 | Ectomomyrmex javanus | Ponerini | Ponerinae | P/s | 470.2852 | 95.8 | 98.5 | 1.82 | Final |
| GAGA-0266 | Ectomomyrmex cf. javanus sp2 | Ponerini | Ponerinae | P/s | 433.5352 | 93.4 | 96.5 | 0.81 | Final |
| GAGA-0307 | Hypoponera opacior | Ponerini | Ponerinae | P/s | 250.7967 | 97.0 | 98.5 | 6.95 | Final |
| NCBI-0009 | Harpegnathos saltator | Ponerini | Ponerinae | non-GAGA | 334.5273 | 97.3 | 98.1 | 1.08 | NCBI assembly |
| GAGA-0303 | Harpegnathos venator | Ponerini | Ponerinae | P/s | 323.9676 | 97.2 | 98.5 | 3.00 | Final |
| GAGA-0301 | Anochetus risii | Ponerini | Ponerinae | P/s | 438.2270 | 94.5 | 96.9 | 4.72 | Final |
| GAGA-0353 | Odontomachus cf. monticola | Ponerini | Ponerinae | P/s | 594.5146 | 95.5 | 97.3 | 2.69 | Final |
| NCBI-0011 | Odontomachus brunneus | Ponerini | Ponerinae | non-GAGA | 393.0181 | 96.5 | 98.9 | 1.76 | NCBI assembly |
| GAGA-0074 | Odontomachus hastatus | Ponerini | Ponerinae | P/s | 411.5903 | 96.4 | 99.2 | 3.13 | Final |
| GAGA-0386 | Euponera pilosior | Ponerini | Ponerinae | stLFR | 397.3314 | 84.2 | 96.5 | 0.05 | Final |
| GAGA-0408 | Buniapone amblyops | Ponerini | Ponerinae | stLFR | 306.4234 | 94.3 | 98.1 | 0.12 | Final |
| GAGA-0246 | Pseudoneoponera rufipes | Ponerini | Ponerinae | P/s | 305.0238 | 96.8 | 97.7 | 7.76 | Final |
| GAGA-0387 | Leptogenys cf. binghamii | Ponerini | Ponerinae | stLFR | 265.4717 | 89.3 | 90.6 | 0.08 | Final |
| GAGA-0352 | Leptogenys diminuta | Ponerini | Ponerinae | P/s | 347.5120 | 97.1 | 97.3 | 9.63 | Final |
| GAGA-0380 | Leptogenys cf. kitteli | Ponerini | Ponerinae | P/s | 318.0849 | 97.1 | 98.5 | 6.35 | Final |
| GAGA-0025 | Megaponera analis | Ponerini | Ponerinae | P/s | 312.3925 | 96.4 | 98.1 | 6.45 | Final |
| GAGA-0536 | Prionopelta kraepelini | Amblyoponini | Amblyoponinae | stLFR | 242.7429 | 75.6 | 79.6 | 0.05 | Final |
| GAGA-0391 | Stigmatomma cf. rubiginoum | Amblyoponini | Amblyoponinae | P/s | 285.9319 | 95.1 | 96.9 | 5.42 | Final |
| GAGA-0404 | Mystrium cf. camillae | Amblyoponini | Amblyoponinae | P/s | 289.9689 | 96.2 | 95.6 | 6.05 | Final |
| GAGA-0552 | Paraponera clavata | tr. Paraponerinae | Paraponerinae | P/s | 226.7042 | 96.2 | 95.6 | 6.18 | Final |
| GAGA-0535 | Discothyrea kamiteta | Proceratiini | Proceratiinae | stLFR | 233.3178 | 84.1 | 91.8 | 0.02 | Final |
| GAGA-0580 | Probolomyrmex longiscapus | Probolomyrmecini | Proceratiinae | stLFR | 315.5639 | 79.8 | 87.9 | 0.86 | Final |
| GAGA-0389 | Proceratium itoi | Proceratiini | Proceratiinae | stLFR | 308.3117 | 81.7 | 87.9 | 0.02 | Final |
| GAGA-0537 | Proceratium cf. bruelheidei | Proceratiini | Proceratiinae | stLFR | 307.8773 | 83.0 | 86.7 | 0.02 | Final |